Nauka Przyroda Technologie

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2015 volume 9 issue 3, #34 DOI: 10.17306/J.NPT.2015.3.34
Aleksandra Wawro, Adam Rzeszutek, Żaneta Bartkowiak, Dominika Pieprzyk-Kokocha, Włodzimierz Grajek
Optimization of chemical mutagenesis of yeast Saccharomyces cerevisiae using ethyl methanesulfonate (EMS)
Summary.

This paper attempts to optimize the methods of chemical mutagenesis of distillers yeast Saccharomyces cerevisiae using an alkylating agent ethyl methanesulfonate (EMS). Several rounds of mutagenesis were carried out, mutagen concentration and duration of exposure were adopted as variables. Optimal conditions required to ensure the death of the yeast cells were achieved during the log phase of cell, at concentration of mutagen 114.5–137.4 mg/cm3 and during the mutagenesis duration exposure time 60 min. As a result, several tens of yeast mutants were obtained. Based on testing of fermentation were selected five mutants characterized by a higher volumetric ethanol productivity than the starting strain. The obtained mutants were subjected to the screening test for their resistance to acetic acid. It was found that the mutants showed two times higher resistance to acetic acid than the starting strain.

Key words: yeast, Saccharomyces cerevisiae, chemical mutagenesis, EMS, the volumetric ethanol productivity (Pv)
PDFFull text available in Polish in Adobe Acrobat format:
http://www.npt.up-poznan.net/tom9/zeszyt3/art_34.pdf

DOI: 10.17306/J.NPT.2015.3.34

For citation:

MLA Wawro, Aleksandra, et al. "Optymalizacja mutagenizacji chemicznej drożdży Saccharomyces cerevisiae z użyciem metanosulfonianu etylu (EMS)." Nauka Przyr. Technol. 9.3 (2015): #34. DOI: 10.17306/J.NPT.2015.3.34
APA Aleksandra Wawro1, Adam Rzeszutek2, Żaneta Bartkowiak2, Dominika Pieprzyk-Kokocha1, Włodzimierz Grajek2 (2015). Optymalizacja mutagenizacji chemicznej drożdży Saccharomyces cerevisiae z użyciem metanosulfonianu etylu (EMS). Nauka Przyr. Technol. 9 (3), #34 DOI: 10.17306/J.NPT.2015.3.34
ISO 690 WAWRO, Aleksandra, et al. Optymalizacja mutagenizacji chemicznej drożdży Saccharomyces cerevisiae z użyciem metanosulfonianu etylu (EMS). Nauka Przyr. Technol., 2015, 9.3: #34. DOI: 10.17306/J.NPT.2015.3.34
Corresponding address:
Aleksandra Wawro
Zakład Innowacyjnych Biomateriałów i Nanotechnologii
Instytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu
ul. Wojska Polskiego 71 B
60-630 Poznań
Poland
e-mail: aleksandra.wawro@iwnirz.pl
Accepted for print: 23.02.2015