Nauka Przyroda Technologie

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Agnieszka Tomkowiak, Dorota Weigt, Alicja Szabelska, Joanna Zyprych, Zbigniew Broda, Idzi Siatkowski
Analysis of genetic diversity of cultivars and clones of white clover (Trifolium repens L.) with the use of RAPD markers
Summary.

The research focused on four white clover cultivars (which are synthetic clones) and one cultivar generatively propagated through the selection-propagation school. The overall objective of this study was to analyse the genetic diversity of cultivars and clones of white clover and to determine the share of individual clones in the creation of cultivars based on genetic similarity specified using RAPD molecular markers. For each similarity coefficient the similarity matrix was created. Then the dendrograms were built. For the results comparison for each ratio Mantel test and Spearman correlation coefficient were used. On the basis of the analysis it was stated that selected primers generated polymorphisms, which allowed to select components for the crosses to test the ability of combinations. The dendrograms constructed with the use of the coefficients of Nei and Ochiai create similarity groups which include cultivars with their clones so they the most precisely group the forms in terms of the origin. Coefficients of Simple Matching, Hamann, Rogers and Tanimoto create groups, which often contain cultivars and clones that do not belong to a given cultivar, so they are not so useful in selecting components for the crosses.

Key words: clover, RAPD markers, genetic similarity
PDFFull text available in Polish in Adobe Acrobat format:
http://www.npt.up-poznan.net/tom6/zeszyt4/art_79.pdf

For citation:

MLA Tomkowiak, Agnieszka, et al. "Analiza zróżnicowania genetycznego odmian i klonów koniczyny białej (Trifolium repens L.) z użyciem markerów RAPD." Nauka Przyr. Technol. 6.4 (2012): #79.
APA Agnieszka Tomkowiak1, Dorota Weigt1, Alicja Szabelska2, Joanna Zyprych2, Zbigniew Broda1, Idzi Siatkowski2 (2012). Analiza zróżnicowania genetycznego odmian i klonów koniczyny białej (Trifolium repens L.) z użyciem markerów RAPD. Nauka Przyr. Technol. 6 (4), #79
ISO 690 TOMKOWIAK, Agnieszka, et al. Analiza zróżnicowania genetycznego odmian i klonów koniczyny białej (Trifolium repens L.) z użyciem markerów RAPD. Nauka Przyr. Technol., 2012, 6.4: #79.
Corresponding address:
Agnieszka Tomkowiak
Katedra Genetyki i Hodowli Roślin
Uniwersytet Przyrodniczy w Poznaniu
ul. Dojazd 11
60-632 Poznań
Poland
e-mail: agatom@up.poznan.pl
Accepted for print: 30.08.2012