Nauka Przyroda Technologie

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2017 volume 11 issue 1, 23-32 http://dx.doi.org/10.17306/J.NPT.00179
Angelika Kiel, Dorota Weigt, Martyna Karpińska, Jerzy Nawracała, Danuta Kurasiak-Popowska, Agnieszka Tomkowiak, Bogusława Ługowska
The use of molecular markers in the selection of wheat genotypes obtained from in vitro cultures
Summary.

Background. Leaf rust caused by Puccinia recondita f. sp. tritici is one of the most dangerous fungal diseases as it causes significant loss to common wheat (Triticum aestivum L.) yield. Breeding for resistance is the most effective method of controlling fungal diseases of cereals. Biotechnological methods such as molecular markers and the haploidisation technique shorten the breeding cycle and increase the efficiency of selection. The aim of the study was to identify the leaf rust resistance gene (Lr19) in wheat genotypes obtained in in vitro cultures.

Material and methods. The plant material consisted of haploid plants and spontaneously doubled haploids from generation F1 hybrids, which were obtained by crossing reference genotypes with the Lr19 gene (T36, T39) and well-yielding varieties ‘Ozon’ and ‘Hondia’. Xwmc221 and GB markers were used for identification of the Lr19 gene. 58 genotypes with the haploid number of chromosomes and 39 spontaneously doubled haploid plants were tested.

Results. The presence of Xwmc221 marker (200bp) was observed in 29 haploid plants and in 24 spontaneously doubled haploids. The presence of GB marker (130bp) was observed in 27 genotypes with the haploid number of chromosomes and in 27 spontaneously doubled haploid plants.

Conclusions. The analyses proved that selection based on molecular markers is possible at the haploid level, which enables the reduction of costs and length of the breeding cycle.

Key words: leaf rust, haploids, DNA markers, Lr19, MAS
PDFFull text available in Polish in Adobe Acrobat format:
http://www.npt.up-poznan.net/tom11/zeszyt1/art_2.pdf

http://dx.doi.org/10.17306/J.NPT.00179

For citation:

MLA Kiel, Angelika, et al. "Wykorzystanie markerów molekularnych w selekcji genotypów pszenicy otrzymanych w kulturach in vitro." Nauka Przyr. Technol. 11.1 (2017): 23-32. http://dx.doi.org/10.17306/J.NPT.00179
APA Angelika Kiel1, Dorota Weigt1, Martyna Karpińska1, Jerzy Nawracała1, Danuta Kurasiak-Popowska1, Agnieszka Tomkowiak1, Bogusława Ługowska2 (2017). Wykorzystanie markerów molekularnych w selekcji genotypów pszenicy otrzymanych w kulturach in vitro. Nauka Przyr. Technol. 11 (1), 23-32 http://dx.doi.org/10.17306/J.NPT.00179
ISO 690 KIEL, Angelika, et al. Wykorzystanie markerów molekularnych w selekcji genotypów pszenicy otrzymanych w kulturach in vitro. Nauka Przyr. Technol., 2017, 11.1: 23-32. http://dx.doi.org/10.17306/J.NPT.00179
Corresponding address:
Angelika Kiel
Katedra Genetyki i Hodowli Roślin
Uniwersytet Przyrodniczy w Poznaniu
ul. Dojazd 11
60-637 Poznań
Poland
e-mail: akiel@up.poznan.pl
Accepted for print: 31.03.2017