Nauka Przyroda Technologie

Science Nature Technologies

Scientific journal presents original papers and articles relevant to all aspects of agricultural sciences.

Język polski English
Quick search:

2016 volume 10 issue 2, #24 DOI: 10.17306/J.NPT.2016.2.24
Agnieszka Tomkowiak, Magdalena Manikowska, Danuta Kurasiak-Popowska, Dorota Weigt, Jerzy Nawracała, Sylwia Mikołajczyk, Janetta Niemann
Identification of the gene Rht8 with using of GWM 261 marker in forms of common wheat with diverse origins
Summary.

Lodging is the most important of the various factors affecting yielding in common wheat (Triticum aestivum L. ssp. vulgare). In breeding one of the most effective methods to obtain cultivars resistant to lodging is to introduce semi-dwarf genes. One of the genes used in wheat breeding programs worldwide is the Rht8 gene, whose presence is correlated with a reduction of stalk length by approximately 10%. This gene is sensitive to the action of exogenous gibberellic acid, thus it may be identified using microsatellite single sequence repeat (SSR) markers. The aim of this study was to identify the Rht8 gene using the GWM 261 marker in 15 winter wheat cultivars of various pedigree. The analyses showed the presence of specific products of 175 bp in cv. ‘Augusta’ and 165 bp in cvs. ‘Brevor’, ‘Greer’, ‘Augusta’, ‘Geneva’, ‘Karl’, ‘Pionier 2548’, ‘Pionier 2737W’. This cultivar may be used in breeding programs as a source of the Rht8 gene.

Key words: wheat, semi-dwarf genes, Rht8, SSR markers
PDFFull text available in Polish in Adobe Acrobat format:
http://www.npt.up-poznan.net/tom10/zeszyt2/art_24.pdf

DOI: 10.17306/J.NPT.2016.2.24

For citation:

MLA Tomkowiak, Agnieszka, et al. "Identyfikacja genu Rht8 z wykorzystaniem markera GWM 261 u form pszenicy zwyczajnej o zróżnicowanym pochodzeniu." Nauka Przyr. Technol. 10.2 (2016): #24. DOI: 10.17306/J.NPT.2016.2.24
APA Agnieszka Tomkowiak, Magdalena Manikowska, Danuta Kurasiak-Popowska, Dorota Weigt, Jerzy Nawracała, Sylwia Mikołajczyk, Janetta Niemann (2016). Identyfikacja genu Rht8 z wykorzystaniem markera GWM 261 u form pszenicy zwyczajnej o zróżnicowanym pochodzeniu. Nauka Przyr. Technol. 10 (2), #24 DOI: 10.17306/J.NPT.2016.2.24
ISO 690 TOMKOWIAK, Agnieszka, et al. Identyfikacja genu Rht8 z wykorzystaniem markera GWM 261 u form pszenicy zwyczajnej o zróżnicowanym pochodzeniu. Nauka Przyr. Technol., 2016, 10.2: #24. DOI: 10.17306/J.NPT.2016.2.24
Corresponding address:
Agnieszka Tomkowiak
Katedra Genetyki i Hodowli Roślin
Uniwersytet Przyrodniczy w Poznaniu
ul. Dojazd 11
60-632 Poznań
Poland

e-mail: popowska@up.poznan.pl
Accepted for print: 1.03.2016